Screening and identifying of hepatoprotective compounds in paeoniae radix rubra / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 597-600, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-274296
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new rapid method to screen potential hepatoprotective compounds from traditional Chinese medicine, and identify the hepatoprotective compounds in Paeoniae Radix Rubra.</p><p><b>METHOD</b>Fluorescein diacetate labelled and MTT assay were applied for screening the hepatoprotective fractions on HepG2 cells exposed to galactosamine. The active fractions were analyzed by chromatography coupled with mass spectrometry. Finally, the hepatoprotective effects of the identified compounds were validated by hepatoprotective assay.</p><p><b>RESULT</b>Three hepatoprotective fractions were founded, in which three compounds were identified as paeoniflorin, ethyl palmitate and ethyl linoleate. Validation results indicated that all the three compounds can attenuate the galactosamine induced injury on HepG2 cells.</p><p><b>CONCLUSION</b>Paeoniflorin, ethyl palmitate and ethyl linoleate from paeoniae radix rubra showed potential hepatoprotective activity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ácidos Palmíticos
/
Farmacologia
/
Benzoatos
/
Hidrocarbonetos Aromáticos com Pontes
/
Ácidos Linoleicos
/
Química
/
Substâncias Protetoras
/
Paeonia
/
Monoterpenos
/
Células Hep G2
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
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