Simultaneous determination of four compounds in Erzhiwan by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 594-596, 2012.
Article
em Zh
| WPRIM
| ID: wpr-274297
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC method for simultaneous determination of nuzhenide, specnuezhenide, wedelolactone and oleanic acid in Erzhiwan.</p><p><b>METHOD</b>The DIKMA C18 (4.6 mm x 200 mm, 5 microm) column was adopted with acetonitrile and 0.1% phosphoric acid solution as the mobile phase and gradient elution. The flow rate was 1.0 mL x min(-1) and the volume of injection was 20 microL. The column temperature was maintained at 30 degrees C and the detective wavelength was set at 215 nm.</p><p><b>RESULT</b>There were good linear relationships between the peak area and concentration at the range of 2.008-80.32 (r = 0.999 6), 5.872-234.88 (r = 0.999 7) , 0.9-36 (r = 0.999 9), 13.24-529.6 mg x L(-1) (r = 0.999 6) for nuzhenide, specnuezhenide, wedelolactone and oleanic acid, respectively. The average recovery rates of nuzhenide, specnuezhenide, wedelolactone and oleanic acid were 99.25%, 98.70%, 96.23% and 101.5%, respectively, with RSD of less than 3%.</p><p><b>CONCLUSION</b>The established method was so easy, rapid and accurate that it can be used as an effective way for quality control of Erzhiwan.</p>
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Assunto principal:
Piranos
/
Medicamentos de Ervas Chinesas
/
Cromatografia Líquida de Alta Pressão
/
Cumarínicos
/
Glucosídeos
/
Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Article