AFLP analysis of genetic diversity of Evodia rutaecarpa / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 278-282, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-274360
ABSTRACT
<p><b>OBJECTIVE</b>To study the genetic background of Evodia rutaecarpa by AFLP, and analyze the genetic diversity of E. rutaecarpa from different areas.</p><p><b>METHOD</b>E. rutaecarpa genomic DNA was extracted. The AFLP reaction system was established and AFLP primer pairs were chosen for the analysis. Forty-six individuals of E. rutaecarpa which from five provinces were analyzed by AFLP. The NTSYS-pc 2.1 software was used for cluster analysis.</p><p><b>RESULT</b>Six out of the original 72 pairs of primers were optimized for the study; AFLP analysis revealed the similarity coefficient of 0.53, the samples of E. rutaecarpa var. officinalis from Zhejiang province was separated from other accessions; E. rutaecarpa var. officinalis also showed more pronounced genetic variation than the E. rutaecarpa, and strong geo-related relevance.</p><p><b>CONCLUSION</b>Variance of genetic background of E. rutaecarpa are large, AFLP analysis method can obviously identify different varieties of E. rutaecarpa, and can detect the genetic characteristics of inter-regional differences.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Variação Genética
/
Classificação
/
DNA de Plantas
/
Evodia
/
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
/
Genética
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
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