Effect of cytoskeleton integrity on the expression of c-fos in osteoblasts induced by fluid shear stress / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 681-685, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-274515
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cytoskeleton integrity on the expression of c-fos gene in osteoblasts induced by fluid shear stress.</p><p><b>METHODS</b>BALB/c mouse primary osteoblasts were divided into four groups (according to fluid shear stress loaded or not and cytochalasin D used or not). The Tagman probe real-time PCR and immunofluorescence were performed to detect the expression levels of c-fos mRNA, c-fos protein and cytoskeleton, respectively. The data were analysed using two-way ANOVA.</p><p><b>RESULTS</b>In control group and cytochalasin D group, fluid shear stress could significantly increase the expression levels of c-fos mRNA (0.1637 +/- 0.0303 and 0.0104 +/- 0.0070, respectively) and protein (177.14 +/- 9.37 and 150.95 +/- 6.17, respectively) in osteoblasts, compared with the unloaded osteoblasts of the control group and the cytochalasin D group (0.0057 +/- 0.0021 and 0.0032 +/- 0.0014, respectively for c-fos mRNA, and 117.96 +/- 4.11 and 119.77 +/- 5.19, respectively for protein, P < 0.05). Induced by the fluid shear stress, the expression levels of c-fos mRNA and protein in cytochalasin D group were lower than control group, and the difference had statistical significance (P < 0.05).</p><p><b>CONCLUSIONS</b>The cytoskeleton integrity in osteoblasts was essential to the expression of c-fos gene induced by fluid shear stress.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Fisiologia
/
Reologia
/
Estresse Mecânico
/
Citoesqueleto
/
RNA Mensageiro
/
Citocalasina D
/
Células Cultivadas
/
Análise de Variância
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS