Characterization of human anti-BAFF scFv-Fc that inhibits the activity of BAFF in vivo / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1336-1340, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-274657
ABSTRACT
To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Baço
/
Sangue
/
Peso Corporal
/
Proteínas Recombinantes de Fusão
/
Imunoglobulina G
/
Imunoglobulina M
/
Fragmentos Fc das Imunoglobulinas
/
Linfócitos B
/
Distribuição Aleatória
/
Diferenciação Celular
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2012
Tipo de documento:
Artigo
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