Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1159-1163, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-274683
ABSTRACT
Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antivirais
/
Farmacologia
/
Plasmídeos
/
Ribavirina
/
RNA Polimerase Dependente de RNA
/
Transfecção
/
Amantadina
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Genes Reporter
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2012
Tipo de documento:
Artigo
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