Identification of oligopeptides binding to Mycoplasma hyorhinis P37 using a phage display library / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1165-1188, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-274933
ABSTRACT
Phage display random heptapeptide library was screened with recombinant P37 in this study. The positive phage clones were identified by ELISA and were sequenced, and the amino acid sequences of the polypeptides displayed on phage were deduced. After GST-polypeptides fusion protein was constructed and expressed, its binding to P37 was determined by GST-pull down and Western blot. After 4 rounds of bio-panning, the enriched positive phage clones were identified by ELISA. Eighteen positive phage clones were sequenced and the peptide sequences were as follows. ACAPKPPWLC (12/18), RPLSIDPWSPHL (3/18), RPLSNDPWSPHL (1/18), QNMMSPIEGVRI (1/ 18) and WAPEKDYMQLMK (1/18). The results from GST-pull down and Western blot showed that peptide RPLSIDPWSPHL could interact with P37. The study will be helpful for identifying the protein reacting with P37.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligopeptídeos
/
Ligação Proteica
/
Suínos
/
Proteínas de Bactérias
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Biblioteca de Peptídeos
/
Mycoplasma hyorhinis
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2011
Tipo de documento:
Artigo
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