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Identification of glucose-responsive elements in the promoter of UDP-L-rhamnose biosynthesis gene RHM1 in Arabidopsis thaliana / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1531-1537, 2008.
Artigo em Chinês | WPRIM | ID: wpr-275326
ABSTRACT
In plants, UDP-L-rhamnose is one of the major components of cell wall skeleton. Rhamnose synthase plays a key role in rhamnose synthesis which converts UDP-D-glucose into UDP-L-rhamnose in plants. In this study, we isolated the 1058 bp promoter region of the rhamnose synthase gene AtRHM1 from Arabidopsis genome by PCR, and created a series of deletions of AtRHM1 promoter ranging from -931 bp to +127 bp. The full length of the promoter and its deletion derivatives fused with GUS reporter gene were introduced into wild-type Arabidopsis by Agrobacterium-mediated transformation respectively. The GUS staining and GUS enzymatic activity assay showed that the expression of AtRHM1 is induced at transcriptional level by glucose and the regulatory elements involved in the glucose response are located in the region of -931 bp - -752 bp which contains three G-box motifs.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Uridina Difosfato Glucose / Açúcares de Uridina Difosfato / Regiões Promotoras Genéticas / Plantas Geneticamente Modificadas / Arabidopsis / Proteínas de Arabidopsis / Genética / Glucosiltransferases / Metabolismo Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Uridina Difosfato Glucose / Açúcares de Uridina Difosfato / Regiões Promotoras Genéticas / Plantas Geneticamente Modificadas / Arabidopsis / Proteínas de Arabidopsis / Genética / Glucosiltransferases / Metabolismo Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2008 Tipo de documento: Artigo