Cloning, expression and identification of IL-1ra-Fcepsilon fusion gene / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1754-1760, 2008.
Article
em Zh
| WPRIM
| ID: wpr-275344
Biblioteca responsável:
WPRO
ABSTRACT
Both interleukin-1 and IgE are important in the pathogenic mechanism of the allergy asthma. cDNA of interleukin-1receptor antagonist (IL-1ra) and IgE were cloned and a prokaryotic expression vector IL-1ra-Fcepsilon/pBV220 was constructed. The vector was transformed into Escherichia coli BL21(DE3). The fusion protein was expressed successfully in the form of inclusion body. The recombination protein of IL-1ra-Fcepsilon was highly purified by chromatography of gel filtration and ion exchange, which was identifited by Western blotting. The cell assay showed that the activity of IL-1ra-Fcepsilon was as high as IL-1ra in vitro after refolding. The pharmacokenetic profile of IL-1ra-Fcepsilon and L-1ra was analyzed, and the half time of IL-1ra-Fcepsilon is 4.78 times than that of IL-1ra.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Proteínas Recombinantes de Fusão
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Imunoglobulina E
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Fragmentos Fc das Imunoglobulinas
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Clonagem Molecular
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Escherichia coli
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Fusão Gênica
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Proteína Antagonista do Receptor de Interleucina 1
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Genética
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Metabolismo
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Article