Effect on production of avermectins of spore pigment biosynthesis in Streptomyces avermitilis NRRL8165 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1702-1706, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-275352
ABSTRACT
The flanking fragments of the whiE(a) gene cluster was PCR amplified, cloned and used to construct the gene replacement plasmid pHL643. pHL643 was conjugated into Streptomyces avermitilis NRRL8165 followed by screening for double crossover event, yielding three apramycin resistance and thiostrepton sensitive isolates named ZJ1, ZJ2 and ZJ3, which were deficient in biosynthesis of the grey spore pigment. The whiE(a) gene replacement of these isolates was confirmed by Southern hybridization. Fermentation of the mutant strains in shaking flasks and HPLC analyses showed that the production of avermectins increased by 47% compared with that of the wild type, indicating that the spore pigment biosynthesis competes with the avermectins biosynthetic pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeos
/
Esporos Bacterianos
/
Streptomyces
/
Proteínas de Bactérias
/
Ivermectina
/
Clonagem Molecular
/
Biologia Celular
/
Genética
/
Metabolismo
/
Mutação
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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