Selection of a novel single-chain variable fragment antibody specifically against a linear epitope of white spot syndrome virus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1387-1394, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-275373
ABSTRACT
White spot syndrome virus (WSSV) is one of the most important pathogens in shrimp farm throughout the world. Many researches on WSSV have been done, but no efficient approach has been gained to protect and cure the disease. In this study, we constructed a single-chain fragment variable (scFv) antibody library displayed on phage using spleen cells from mice immunized with denatured WSSV. After several rounds of panning respectively against purified intact WSSV virions and purified VP28 expressed in Escherichia coli, five novel scFv antibodies specifically against WSSV were selected, one of which, clone P75E8, recognized a linear epitope. The location in virions of the epitopes recognized by the five scFv clones was determined by immunoelectron microscopy. This study provides a new way to obtain more different antibodies specifically binding to WSSV, and especially provides a new strategy to obtain scFvs against linear epitopes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Região Variável de Imunoglobulina
/
Fragmentos de Imunoglobulinas
/
Dados de Sequência Molecular
/
Sequência de Aminoácidos
/
Biblioteca de Peptídeos
/
Penaeidae
/
Vírus da Síndrome da Mancha Branca 1
/
Alergia e Imunologia
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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