Effect of solution environment on the purification of pertussis toxin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1279-1284, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-275389
ABSTRACT
The low recovery of pertussis toxin (PT) and the low resolving efficiency of chromatography, due to the instability of PT in low salt condition, are the main challenges for its purification. We aplied 2 mol/L urea to prevent the aggregation and disassociation of PT during the purification by ion-exchange chromatography (IEC) and gel filtration chromatography (GFC). The effect of urea on the purification of PT was studied by ELISA assay and non-reduced SDS-PAGE. The activity recoveries of PT and filamentous hemagglutinin (FHA) in IEC and GFC, the resolution efficiency in GFC and the purities of PT and FHA were improved obviously by adding 2 mol/L urea in the mobile phase. The results highlight the potential application of urea in the acellular pertussis vaccine (APV) manufacture procedure.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Soluções
/
Ureia
/
Fatores de Virulência de Bordetella
/
Vacina contra Coqueluche
/
Química
/
Cromatografia por Troca Iônica
/
Adesinas Bacterianas
/
Vacinas Acelulares
/
Toxina Pertussis
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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