Production and accumulation of xylooligosaccharides with long chains by growing culture and xylanase of a mutant strain of Bacillus pumilus X-6-19 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1221-1227, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-275399
ABSTRACT
Bacillus pumilus X-6-9 isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established, when compared to the original culture conditions of B. pumilus X-6-19. The addition of D-glucose to the culture of the mutant strain U-3 of B. pumilus X-6-9 repressed the synthesis of beta-xylosidase, but not xylanase. Thus, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharides with long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized. The hydrolyzates generated by the purified xylanase contained xylobiose, xylotriose, xylotetraose, and xylopentaose, but not xylose.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligossacarídeos
/
Farmacologia
/
Microbiologia do Solo
/
Bacillus
/
Proteínas Recombinantes
/
Química
/
Endo-1,4-beta-Xilanases
/
Técnicas de Cultura
/
Genética
/
Glucose
Idioma:
Inglês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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