Na (+) /Ca (2+) Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 3083-3087, 2015.
Article
em En
| WPRIM
| ID: wpr-275563
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Na + /Ca 2+ exchanger (NCX) plays a crucial role in pentylenetetrazol-induced convulsion. However, it is unclear whether NCX is critically involved in hyperthermia-induced convulsion. In this study, we examined the potential changes in NCX3 in the hippocampus and cerebrocortex of rats with hyperthermia-induced convulsion.</p><p><b>METHODS</b>Twenty-one Sprague Dawley rats were randomly assigned to control group, convulsion-prone group and convulsion-resistant group (n = 7 in each group). Whole-cell patch-clamp method was used to record NCX currents. Both the Western blotting analysis and immunofluorescence labeling techniques were used to examine the expression of NCX3.</p><p><b>RESULTS</b>NCX currents were decreased in rats after febrile convulsion. Compared to the control group, NCX3 expression was decreased by about 40% and 50% in the hippocampus and cerebrocortex of convulsion-prone rats, respectively. Furthermore, the extent of reduction in NCX3 expression seemed to correlate with the number of seizures.</p><p><b>CONCLUSIONS</b>There is a significant reduction in NCX3 expression in rats with febrile convulsions. Our findings also indicate a potential link between NCX3 expression, febrile convulsion in early childhood, and adult onset of epilepsy.</p>
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Índice:
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Assunto principal:
Convulsões
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Regulação para Baixo
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Córtex Cerebral
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Ratos Sprague-Dawley
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Trocador de Sódio e Cálcio
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Febre
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Hipocampo
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Metabolismo
Limite:
Animals
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Pregnancy
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2015
Tipo de documento:
Article