Study on sperm damage caused by trichloroethylene in male rats / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 811-814, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-275809
ABSTRACT
<p><b>OBJECTIVE</b>To study in vitro sperm damage caused by trichloroethylene in male rats.</p><p><b>METHODS</b>Sperms of Sprague-Dawley (SD) rats were collected 4 hours after being contaminated by trichloroethylene of 0, 2, 4, 6, 8, and 10 mmol/L in vitro. Giemsa staining was performed to observe the morphological changes of sperms, and flow cytometer was used to detect the changes in mitochondrial membrane potential.</p><p><b>RESULTS</b>The sperm motilities in 6, 8, and 10 mmol/L trichloroethylene groups decreased significantly compared with that in control group (P <0.01); the sperm aberration rates in 8 and 10 mmol/L trichloroethylene groups were significantly higher than that in control group (P<0.01). With the increase in exposure dose, the proportion of sperms with reduced mitochondrial membrane potential increased, and there were significant differences in sperm apoptosis rate between the 4, 6, 8, and 10 mmol/L trichloroethylene groups and control group (P<0.01).</p><p><b>CONCLUSION</b>In vitro exposure to trichloroethylene can reduce sperm motility and increase the aberration rate and apoptosis rate of sperms in male SD rats.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Motilidade dos Espermatozoides
/
Espermatozoides
/
Tricloroetileno
/
Ratos Sprague-Dawley
/
Apoptose
/
Biologia Celular
/
Toxicidade
/
Potencial da Membrana Mitocondrial
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2013
Tipo de documento:
Artigo
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