Effect of 0.1 Gy X-ray irradiation on gene expression profiles in normal human lymphoblastoid cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 749-752, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-275821
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of 0.1 Gy X-ray irradiation on the gene expression profiles in normal human lymphoblastoid cells using gene microarray and to explore the possible mechanism of the biological effect of low-dose irradiation.</p><p><b>METHODS</b>The NimbleGen 12×135 K microarray corresponding to 45033 genes was used to analyze the gene expression profiles in AHH-1 cells cultured for 6 h and 20 h after 0.1 Gy X-ray irradiation. A gene was identified as the differentially expressed gene if the ratio between its expression levels in irradiation group and control group was higher than 2 or lower than 0.5. RT-PCR and real-time PCR were used to confirm some differentially expressed genes.</p><p><b>RESULTS</b>There were 760 up-regulated genes and 1222 down-regulated genes in the cells at 6 h after 0.1 Gy X-ray irradiation, while there were 463 up-regulated genes and 753 down-regulated genes at 20 h after 0.1 Gy X-ray irradiation; there were 92 differentially expressed genes in common. The expression of GADD45A, CDKN2A, and Cx43 measured using gene microarray was confirmed by RT-PCR and real-time PCR.</p><p><b>CONCLUSION</b>Low-dose irradiation can affect the expression of many functional genes, which provides a basis for the research on the mechanism of radiation damage.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doses de Radiação
/
Efeitos da Radiação
/
Radiação Ionizante
/
Raios X
/
Linfócitos
/
Linhagem Celular
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Transcriptoma
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2013
Tipo de documento:
Artigo
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