Method for determining brodifacoum in workplace air by high-performance liquid chromatography / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 625-626, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-275868
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for determining brodifacoum in workplace air by high-performance liquid chromatography (HPLC).</p><p><b>METHODS</b>Brodifacoum in workplace air was collected with a polytetrafluoroethylene filter and desorbed by mixed solution of methanol and dichloromethane (2080, VV), and was then separated using an ODS column and determined by an ultraviolet detector; retention time was used for identification, and peak area was used for quantification.</p><p><b>RESULTS</b>The concentration of brodifacoum showed a linear relationship with peak area within 0.2∼10.0 µg/ml; the elution efficiency was 91.6%∼95.1%; the detection limit was 0.08 µg/ml (injection volume 20 µl eluate); the minimum detectable concentration was 0.000 67 mg/m(3) (calculated by 240 L air sample).</p><p><b>CONCLUSION</b>This HPLC method is convenient and simple for air collection and sample preparation and meets the methodological requirements. Therefore, this method can be used for the determination of brodifacoum in workplace air.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Local de Trabalho
/
Ar
/
Poluentes Ocupacionais do Ar
/
4-Hidroxicumarinas
/
Métodos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2013
Tipo de documento:
Artigo
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