An optimal method for cryopreservation of microamount round spermatids of the mouse / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To search for an optimal protocol and freezing conditions for the cryopreservation of microamount round spermatids of the mouse.</p><p><b>METHODS</b>We compared the survival rates of frozen-thawed microamount round spermatids of the mouse achieved by vitrification or standard slow freezing with different concentrations of glycerol (5, 7, or 9%) and different lengths of equilibrium time (0, 15, 30, 45, or 60 min).</p><p><b>RESULTS</b>Under the conditions of 7% glycerol and 30 min equilibrium, both vitrification and standard slow freezing achieved high survival rates of spermatids, and the former obtained an even higher rate than the latter ([72.9 ± 15.4]% vs [58.2 ± 17.7]%, P < 0.05).</p><p><b>CONCLUSION</b>A high rate of frozen-thawed microamount round spermatids of the mouse can be achieved by vitrification under the conditions of 7% glycerol and 30 min equilibrium.</p>