Expression of ZEB1 in the prepuce of hypospadias children and its implication / 中华男科学杂志
National Journal of Andrology
;
(12): 510-514, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-276067
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of zinc finger E-box binding homebox 1 (ZEB1) in the prepuce of hypospadias children and its relationship to the incidence of hypospadias.</p><p><b>METHODS</b>Prepuce tissues were collected from 37 children aged 6-15 months undergoing hypospadias repair and 11 age-matched controls receiving circumcision. Based on the position of the urethral meatus, the hypospadias cases were classified as severe (n = 13) and mild-moderate (n = 24). The mRNA and protein expressions of ZEB1 were determined by immunohistochemistry and RT-PCR.</p><p><b>RESULTS</b>The expression of the ZEB1 protein was remarkably higher in the severe (100% [13/13]) and mild-moderate hypospadias patients (75.0% [18/24]) than in the controls (9.1% [1/11]), with statistically significant differences between any two groups (P < 0.05). RT-PCR showed the integrated density value (IDV) of the ZEB1 mRNA expression to be (0.67 ± 0.21), (0.81 ± 0.24), and (1.55 ± 0.29) in the control, mild-moderate, and severe hypospadias patients, respectively, significantly higher in the severe hypospadias than in the control and mild-moderate hypospadias groups (P < 0.05), but with no significant difference between the latter two (P = 0.64).</p><p><b>CONCLUSION</b>The expression of ZEB1 is significantly increased in hypospadias patients, and its upregulation is positively correlated with the severity of hypospadias, which suggests that the overexpression of ZEB1 may contribute to the development of hypospadias.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pênis
/
Fatores de Transcrição
/
Uretra
/
RNA Mensageiro
/
Imuno-Histoquímica
/
Biomarcadores
/
Estudos de Casos e Controles
/
Regulação para Cima
/
Circuncisão Masculina
/
Classificação
Tipo de estudo:
Estudo observacional
/
Fatores de risco
Limite:
Humanos
/
Lactente
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2015
Tipo de documento:
Artigo
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