Preparation of anti-hNOK antibodies and expression examination of NOK in the lung cancer tissues / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 480-484, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-276097
ABSTRACT
Novel Oncogene with Kinase-domain (NOK) is a novel tumor-related gene, coding receptor like protein with a kinase domain. Overexpression of NOK leads to tumorigenesis and metastasis. To further study NOK function in physiological condition, it is necessary to prepare the anti-NOK antibody. In this report, GST fusion protein was adopted to prepare polyclonal antibodies against hNOK. The result showed that the antibodies we generated is with a very high titriation, and can be used for examination of NOK protein by Westernblot. Furthermore, the antibodies were used for immunohistochemistry in lung cancer tissues, and the results demonstrated high expression of hNOK in the tumor tissues. The antibody of hNOK we generated can serve as a diagnostic method for the lung cancer.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Biomarcadores Tumorais
/
Proteínas Oncogênicas
/
Receptores Proteína Tirosina Quinases
/
Diagnóstico
/
Alergia e Imunologia
/
Genética
/
Neoplasias Pulmonares
/
Metabolismo
/
Anticorpos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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