Construction of a novel gene therapy lentiviral vector for drug resistant selection and detection in vivo / 生物工程学报
Chinese Journal of Biotechnology
; (12): 256-261, 2008.
Article
em Zh
| WPRIM
| ID: wpr-276130
Biblioteca responsável:
WPRO
ABSTRACT
Lentiviral vectors were powerful gene delivery tools for gene therapy. We developed a new lentiviral vector pBobi-MIL that constitutively expressed O6-methylguanine-DNAmethyltransferase (MGMT) and Luciferase, linked by the internal ribosomal entry site (IRES), to realize drug tolerance and real time monitoring in vivo. All results from RT-PCR, drug treating clones forming, immunofluorometric assay and chemiluminescence detection showed that cells infected by recombinant lentivirus L-MIL simultaneously expressed these two genes. This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Resistência a Medicamentos
/
Metilases de Modificação do DNA
/
Terapia Genética
/
Lentivirus
/
Proteínas Supressoras de Tumor
/
Enzimas Reparadoras do DNA
/
Vetores Genéticos
/
Genética
/
Luciferases
/
Metabolismo
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Article