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Preparation of metal chelate affinity chromatographic medium and its application in the purification of 6 x histidine-tagged protein / 生物工程学报
Chinese Journal of Biotechnology ; (12): 941-946, 2007.
Artigo em Chinês | WPRIM | ID: wpr-276183
ABSTRACT
Using Sepharose CL-6B as support, 3-Chloro-1, 2-epoxypropane as activated agent, carboxymethylated aspartate (CM-Asp) as chelating ligand, A chelate affinity chromatographic medium based on Co2+, named Co-CM-Asp-Sepharose, was prepared and used to purify 6 x His-tagged fusion proteins. The amount of Co-CM-Asp-Sepharose reacted with 200 microL of lysate, the incubation time, wash condition and the imidazole concentration in the elution buffer were optimized. The purification results using Co-CM-Asp-Sepharose and Ni-NTA-Agarose (product of Qiagen) were compared. The CD155D1 fusion protein was also purified from 5mL of lysate and the amount of protein was determined by Bradford method. The results show that 60 microL of Co-CM-Asp-Sepharose (50% suspension) was suitable for the protein purification from 200 microL of lysate, the optimal incubation time of medium and lysate was 30 min, the optimal imidazole concentration in the eluting buffer was 200 mmol/L, and 200 microg of fusion protein was obtained. In a big scale experiment, 4.6 mg of fusion protein was obtained from 5 mL of lysate using 1.5 mL of Co-CM-Asp-Sepharose (50% suspension). Compared with Ni-NTA-Agarose, the Co-CM-Asp-Sepharose medium exhibits higher selectivity and the protein possesses higher purity.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Polímeros / Sefarose / Proteínas Recombinantes de Fusão / Quelantes / Química / Cromatografia de Afinidade / Ácido Aspártico / Compostos de Epóxi / Genética / Histidina Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Polímeros / Sefarose / Proteínas Recombinantes de Fusão / Quelantes / Química / Cromatografia de Afinidade / Ácido Aspártico / Compostos de Epóxi / Genética / Histidina Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2007 Tipo de documento: Artigo