Analysis of primary metabolites of ranolazine in dog urine by LC-MS(n) / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1031-1038, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-276204
ABSTRACT
Ranolazine and metabolites in dog urine were identified by LC-MS(n). Dog urine samples were collected after ig 30 mg x kg(-1) ranolazine, then the samples were enriched and purified through solid-phase extraction cartridge. The purified samples were analyzed by LC-MS(n). The possible metabolites were discovered by comparing the full scan and SIM chromatograms of the test samples with the corresponding blanks. Seventeen phase I metabolites and fourteen phase II metabolites were identified in dog urine. Three metabolites were identified by comparing with the control article. The metabolites were formed via the following metabolic pathways O-demethylation, O-dearylation, hydroxylation, N-dealkylation, amide hydrolysis, glucuronidation and sulfation. The LC-MS(n) method is suitable for the rapid identification of drug and its metabolites in biologic samples.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Piperazinas
/
Urina
/
Administração Oral
/
Cromatografia Líquida
/
Espectrometria de Massas por Ionização por Electrospray
/
Espectrometria de Massas em Tandem
/
Extração em Fase Sólida
/
Ranolazina
/
Acetanilidas
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2012
Tipo de documento:
Artigo
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