A study on stem cell mobilization after partial liver transplantation in rats / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the role of stem cell mobilization on regeneration of partially grafted livers.</p><p><b>METHODS</b>Rats models with cross-sex 50% PLTx (partial liver transplantation) were established. The rats were divided into three groups PLTx, WLTx (whole liver transplantation) and sham operation groups. Bone marrow and liver samples were collected on days 1, 3, 5, 7 postoperatively (each n = 6). The quantitative variations of the cells with stem cell markers in the bone marrow, including beta2m-/Thy-1.1+, CD45+/CD34+, Flt2/3+ and c-kit+ markers, were detected using flow cytometry. Sry gene positive cells in donor livers were detected by fluorescent in situ hybridization (FISH), and the expressions of CD34, c-kit and Thy-1.1 were detected by immunohistochemistry technique.</p><p><b>RESULTS</b>Compared with the WLTx and sham operation groups, beta2m-/Thy-1.1+, CD45+/CD34+ cells in bone marrows in the PLTx group increased on the first postoperative day and decreased on the following days. The CD34, c-kit and Thy-1.1 positive cells detected in portal tract areas peaked during the 3-5 postoperative days. CD34+/CD45+ positive cells could be detected. The expressions of CD34, c-kit and Thy-1.1 positive cells were rare in the WLTx and sham operation groups. Sry+ cells could be detected in portal tract areas and few Sry+/CD34+ and Sry+/Thy-1.1+cells were detected.</p><p><b>CONCLUSION</b>In the PLTx group, the stem cells in the bone marrow were mobilized and stem cells in the liver were activated.</p>