Genomic analysis of a familial clustering of chronic hepatitis B patients / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 811-814, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-276344
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the peripheral blood monocyte (PBMC) gene expression profile in a familial clustering of patients with chronic hepatitis B (CHB).</p><p><b>METHODS</b>cRNA prepared from PBMC in a family with 5 CHB patients and 4 normal controls was hybridized to high-density oligouncleotide arrays (HG-U133A 2.0 Human GeneChips, Affymetrix), which interrogate the expression of approximately 22,000 human ESTs. Primary image obtained from scanning was analysed with a DNT software package. Real-time PCR was employed to confirm the gene chip results.</p><p><b>RESULTS</b>55 genes out of 22,000 ESTs were identified differently. Among the 55 genes 14 showed increased expression and 41 showed decreased expression in the familial clustering CHB patients compared with those in normal controls. Most of the genes (57%) were involved in immunity, inflammation, apoptosis, signaling transduction, and cell cycle.</p><p><b>CONCLUSION</b>These results suggest that the hosts with this broad range of gene expression alterations are susceptible to hepatic B infection.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Linhagem
/
Leucócitos Mononucleares
/
Análise por Conglomerados
/
Saúde da Família
/
Genoma
/
Hepatite B Crônica
/
Predisposição Genética para Doença
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Genética
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS