Gene expression analysis in liver tissue at a single cell level by nested polymerase chain reaction and laser microdissection / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 30-32, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-276511
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the measurements of gene expressing at a single hepatocyte level.</p><p><b>METHODS</b>Individual hepatocyte was isolated from cryostat tissue section using laser microdissection technique. To detect the mRNA expressed by single hepatocyte, RNA was extracted, reversely transcribed to cDNA and amplified by nested polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Single cell was microdissected from cryostat tissue using an ultraviolet laser micromanipulator. The RNA could be extracted from the isolated cell(s), and the RT-PCR production could be observed after electrophoresis, whose quantitation was compatible with the number of cells.</p><p><b>CONCLUSION</b>Combining laser microdissection and nested RT-PCR can monitor gene expression at a single cell level in vivo.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Perfilação da Expressão Gênica
/
Hepatócitos
/
Dissecação
/
Lasers
/
Metabolismo
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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