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Realgar nano-particles induce apoptosis and necrosis in leukemia cell lines K562 and HL-60 / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 136-140, 2005.
Artigo em Chinês | WPRIM | ID: wpr-276628
ABSTRACT
<p><b>OBJECTIVE</b>To examine the growth-inhibitory, apoptosis- and necrosis-inducing effects of realgar nano-particles (RNP) in human chronic myelogenous leukemia cell line K562 and acute myeloid leukemia cell line HL-60, and to find out the chemical species with efficacy.</p><p><b>METHOD</b>A "solvent-relay" strategy was used for the preparation of RNP suspension. Cell viability was determined by MTT assay. Cell apoptosis and necrosis were characterized with Annexin V-PI double staining in association with flow cytometry and with morphological examination with Hoechst 33258 staining. Parallel experiments with arsenous acid (H3AsO3), the dominant form of arsenic trioxide in the solution, were conducted for comparison.</p><p><b>RESULT</b>The mean diameter of RNP was 159.0 nm. RNP showed growth-inhibitory effect on both cell lines. The double staining test indicated that RNP induced both apoptosis and necrosis, and this was further confirmed by morphological examination.</p><p><b>CONCLUSION</b>RNP induced both apoptosis and necrosis in leukemia cell lines K562 and HL-60. Thioarsenite species with both As-O and As-S bonds may be the active intermediates in the RNP.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Tamanho da Partícula / Patologia / Farmacologia / Arsenicais / Sulfetos / Materia Medica / Apoptose / Células HL-60 / Células K562 / Nanotecnologia Limite: Humanos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Tamanho da Partícula / Patologia / Farmacologia / Arsenicais / Sulfetos / Materia Medica / Apoptose / Células HL-60 / Células K562 / Nanotecnologia Limite: Humanos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2005 Tipo de documento: Artigo