Pseudolaric acid B induces human melanoma A375-S2 cell apoptosis in vitro / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 55-57, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-276644
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells.</p><p><b>METHOD</b>MTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused.</p><p><b>RESULT</b>Pseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased.</p><p><b>CONCLUSION</b>The major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Proteínas
/
Química
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Pinaceae
/
Linhagem Celular Tumoral
/
Diterpenos
/
Relação Dose-Resposta a Droga
Limite:
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2005
Tipo de documento:
Artigo
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