Investigation on the molecular mechanisms of anti-hepatocarcinoma herbs of traditional Chinese medicine by cell cycle microarray / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 50-54, 2005.
Article
em Zh
| WPRIM
| ID: wpr-276645
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To design DNA microarray and investigate the molecular anti-tumor mechanism of herbs of traditional Chinese medicine.</p><p><b>METHOD</b>cDNA microarrays consisting of 56 probes representing 24 human cell cycle genes were constructed, Four anti-hepatocarcinoma herbs including Radix Linderae, Hebra Artemisiae Annuae, Radix Amebiae, Radix Astragli, were chosen. Effects of herbs on SMMC-7721 cell cycle were observed by flow cytometry assay. Effects of herbs on cell cycle gene expression in SMMC-7721 cells were analyzed by comparing hybridization of Dig-Labeled cDNAs from herb-treated cells and cDNAs from untreated cells.</p><p><b>RESULT</b>Expressions of cell cycle geneswere changed in different degrees after herbs treated. Some genes were down-regulated and some genes were up-regulated. The changes in gene expression agreed with the results of flow cytometry assay.</p><p><b>CONCLUSION</b>The results suggest that these herbs may have effects on cell cycle and DNA damage checkpoint genes which may be the mechanism of the herbs, and DNA microarray can be used to investigate the biological function of extracts of traditional Chinese medicine.</p>
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Índice:
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Assunto principal:
Patologia
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Farmacologia
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Plantas Medicinais
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Medicamentos de Ervas Chinesas
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Química
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Amplificação de Genes
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Proteínas Proto-Oncogênicas
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Carcinoma Hepatocelular
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Antígeno Nuclear de Célula em Proliferação
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Genes cdc
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Ano de publicação:
2005
Tipo de documento:
Article