The modulation of pilose antler extract (PAE) on the proliferation of rat osteogenic cells UMR-106 / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 74-77, 2004.
Article
em Zh
| WPRIM
| ID: wpr-276661
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the modulation of pilose antler extract (PAE) on rat osteogenic cells UMR-106 in vitro.</p><p><b>METHOD</b>Component P2 of PAE was isolated by Sephacryl S-200HR gel filtration chromatography. The proliferative effects of P2 and other components isolated by Sephacryl S-200HR on UMR-106 cells were investigated by MTT assay.</p><p><b>RESULT</b>The P2 could significantly increase the proliferation rate of osteogenic cells. When the protein concentration of P2 was between 0.972 mg x L(-1) and 97.2 mg x L(-1), it could inhibit the proliferation of UMR-106 cells. But while the concentration was equal to or greater than 97.2 mg x L(-1), the P2 could increase the proliferation rate of cells, especially 477.92% at 9.72 g x L(-1), which was approximated to 499.62% of PAE. The molecular weight of the P2 was about 59 kDa determined by SDS-PAGE. On the other hand, inhibition was also observed in the sample of the P3, P4 and P5.</p><p><b>CONCLUSION</b>Those regulative factors in PAE which have different molecular weight can affect the proliferation of UMR-106 cells two-wayly. And this adjustment also relies on the dose of those factors. This finding may help us to understand the possible mechanism of Chinese traditional medicine from animal materials.</p>
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Assunto principal:
Chifres de Veado
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Patologia
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Farmacologia
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Extratos de Tecidos
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Neoplasias Ósseas
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Cervos
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Materia Medica
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Osteossarcoma
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Química
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Linhagem Celular Tumoral
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2004
Tipo de documento:
Article