A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 148-153, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-277288
ABSTRACT
Porcine circovirus type 2 (PCV2) is closely related to the postweaning multisystemic wasting syndrome (PMWS). In this study, the pig serum and tissue samples collected from different regions of Hangzhou District in Zhejiang Province of China between 2003 and 2005 were analyzed by enzyme-linked immunosorbent assay (ELISA) for PCV2 antibody and by polymerase chain reaction (PCR) for ORF2 gene. The results show that out of 1250 randomly collected serum samples, 500 sera (40%) were seropositive for PCV2. PCR results demonstrate that Hangzhou PCV2 with more than 50% Chinese PCV2 strains and French PCV2 formed Cluster A. Only one PCV2 from Hangzhou belonged to Cluster B with some other Chinese PCV2 and Netherlands's isolates. Cluster C consisted of PCV2 isolates from China, US, Canada, UK and Germany. The results indicate that the PCV2 infection was widespread in Hangzhou.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Suínos
/
Doenças dos Suínos
/
Ensaio de Imunoadsorção Enzimática
/
Química
/
China
/
Reação em Cadeia da Polimerase
/
Fases de Leitura Aberta
/
Análise de Sequência de DNA
/
Infecções por Circoviridae
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2008
Tipo de documento:
Artigo
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