Fluorescein Diacetate Microplate Assay in Cell Viability Detection / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 710-714, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-277916
ABSTRACT
Objective To investigate the application of the fluorescein diacetate (FDA) microplate assay in cell viability detection. Methods Cells were seeded in a 96-well culture plate until detection. After incubated with FDA,the plate was detected by fluorescence microplate analyzer. The effects of FDA incubation duration,concentration,and other factors on the assay's accuracy and stability were assessed. We also compared the results of FDA with methyl thiazolyl(MTT) in terms of cell numbers and HOinjury. Results Within 0-30 minutes,the fluorescence-cell number coefficient of FDA assay increased with duration and reached 0.99 in 27-30 minutes. The optimum concentration of final FDA in this study was 10-30 μg/ml. On cell viability detection,the result of FDA method was equivalent to MTT method. As to HOinjury assay,the sensitivity of FDA method was superior to MTT on the higher concentration HOtreatment due to a relative shorter duration for detection. Conclusion As a stable and reliable method,FDA is feasible for cell variability detection under varied conditions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Coloração e Rotulagem
/
Bioensaio
/
Sobrevivência Celular
/
Química
/
Fluoresceínas
/
Fluorescência
/
Peróxido de Hidrogênio
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2016
Tipo de documento:
Artigo
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