Potential molecular mechanisms of quercetin-induced heme oxygenase-1 in rat primary hepatocytes / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 865-868, 2013.
Article
em Zh
| WPRIM
| ID: wpr-277981
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possible molecular mechanisms of heme oxygenase-1 (HO-1) induction by quercetin using rat primary hepatocytes.</p><p><b>METHODS</b>Sprague-Dawley rat primary hepatocytes were isolated using a two-step collagenase perfusion technique and treated with quercetin at various doses (25 - 200 mumol/L) and times (2 - 12 h). To investigate the roles of various signaling pathways, the hepatocytes were pre-treated with 50 mumol/L quercetin plus an extracellular signal-regulated kinase (ERK) inhibitor (PD98059 at 10 mumol/L), a p38 inhibitor (SB203580 at 10 mumol/L), a c-Jun N-terminal kinase inhibitor (SP600125 at 10 mumol/L) or a phosphatidylinositol 3-kinase inhibitor (Wortmannin at 1 mumol/L) for 12 h. Changes in the mRNA and protein levels of HO-1 and nuclear factor, E-2 related factor 2 (Nrf2) were detected by RT-PCR and western blotting.</p><p><b>RESULTS</b>After 4 - 12 h of treatment with quercetin at all concentrations, the HO-1 mRNA level in hepatocytes had increased significantly (vs. untreated control cells; all P less than 0.01). The quercetin-induced HO-1 expression and Nrf2 translocation into the nucleolus was inhibited by PD98059.</p><p><b>CONCLUSION</b>Quercetin may induce HO-1 expression via the ERK/Nrf2 signaling transduction pathway.</p>
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Índice:
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Assunto principal:
Farmacologia
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Quercetina
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Transdução de Sinais
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Células Cultivadas
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Ratos Sprague-Dawley
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Hepatócitos
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MAP Quinases Reguladas por Sinal Extracelular
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Inibidores Enzimáticos
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Fator 2 Relacionado a NF-E2
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Cultura Primária de Células
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2013
Tipo de documento:
Article