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Culture and identification of dendritic cells from the peripheral blood of patients with laryngeal squamous cell carcinoma in vitro / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 616-619, 2002.
Artigo em Chinês | WPRIM | ID: wpr-278126
ABSTRACT
<p><b>OBJECTIVE</b>To culture dendritic cells (DC) from peripheral blood of patients with laryngeal carcinoma for therapeutic aid.</p><p><b>METHODS</b>Adherent peripheral blood mononuclear cells from peripheral blood were cultured with 15 ng/ml rhGM-CSF and 7 ng/ml rhIL-4 for one or two weeks. The purity of DC was detected by immunocytochemistry method. The mixed leukocyte reactions stimulated by DC loaded with laryngeal carcinoma antigen were tested by measuring 3H-TdR uptake.</p><p><b>RESULTS</b>A considerable number of suspended cells with spicular or dendritic appearance were observed after 1 week of culture, and their mitochondria were rich in cytoplasm. The positivity of DC was about 30%-60%. DC loaded with laryngeal antigen could induce proliferation of syngeneic T lymphocytes.</p><p><b>CONCLUSION</b>A large number of DC with high purity can be cultured from peripheral blood of patients with laryngeal carcinoma in vitro. It may be used in further experimental studies for clinical applications.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Sangue / Células Dendríticas / Proteínas Recombinantes / Carcinoma de Células Escamosas / Separação Celular / Células Cultivadas / Neoplasias Laríngeas / Fator Estimulador de Colônias de Granulócitos e Macrófagos Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Chinês Revista: Acta Academiae Medicinae Sinicae Ano de publicação: 2002 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Sangue / Células Dendríticas / Proteínas Recombinantes / Carcinoma de Células Escamosas / Separação Celular / Células Cultivadas / Neoplasias Laríngeas / Fator Estimulador de Colônias de Granulócitos e Macrófagos Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Chinês Revista: Acta Academiae Medicinae Sinicae Ano de publicação: 2002 Tipo de documento: Artigo