Expression of rat protamine gene in MEL cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 601-605, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-278129
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of rat protamine (RP) gene in MEL cells and the effect on cell growth.</p><p><b>METHODS</b>Eukaryotic expression plasmid pCR-3.1-RP was constructed and transfected into MEL cells. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.</p><p><b>RESULTS</b>Transfected MEL cells showed lower growth rate, mitotic index and colony-forming rate. Volumes of cells were reduced and reduction of RNA transcription was observed.</p><p><b>CONCLUSION</b>These results suggest that expression of RP in MEL cell may inhibit the cell growth and proliferation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Plasmídeos
/
Células Tumorais Cultivadas
/
Protaminas
/
Transfecção
/
Leucemia Eritroblástica Aguda
/
Divisão Celular
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2002
Tipo de documento:
Artigo
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