Construction of recombinant human CD59 using ICAM-2 promoter for endothelial-specific expression in xenotransplantation / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 588-591, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-278132
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant human CD59 gene containing intercellular adhesion molecule-2 promoter for high level endothelial-specific expression in xenotransplantation.</p><p><b>METHODS</b>ICAM-2 promotor fragment and CD59-intron 1 fragment were produced by PCR from the human blood genome, and then clone these fragments into a pcDNA3-CD59 eukaryotic expression vector which was followed by digestion with the specific restricted endonuclease (for example EcoRI, Hind III). The ICAM-2 promoter and CD59-intron 1 fragments were identified by PCR, and sequencing. The recombinant was then transfected into pig aorta endothelial cells with Lipofection, and the expression was measured by flow cytometer.</p><p><b>RESULTS</b>Products of the sequences measured were in accord with the frames of the gene bank. The expression of the protein of this recombinant was positive.</p><p><b>CONCLUSION</b>The CD59 recombinant gene is constructed successfully, providing a basis for transgenic research.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Transplante Heterólogo
/
Proteínas Recombinantes
/
Endotélio Vascular
/
Transfecção
/
Antígenos CD
/
Moléculas de Adesão Celular
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
/
Clonagem Molecular
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2002
Tipo de documento:
Artigo
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