Effects of hydrogen peroxide on intracellular free Ca2+ content in rat liver oval cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 281-284, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-278182
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects and mechanism of hydrogen peroxide (H2O2) of low concentration on dynamic changes of intracellular free calcium contents ([Ca2+]i) in cultural rat liver oval cells (WB-F344 cells).</p><p><b>METHODS</b>Using Fluo-3/Am as fluorescent indicator of [Ca2+]i and it was measured by laser scanning confocal microscope system.</p><p><b>RESULTS</b>The results showed that (1) A rapid transient spiking of [Ca2+]i occurred after the stimulation of H2O2 of low concentration (800 nmol/L). (2) The [Ca2+]i increase was abolished by pretreated with catalase (CAT) or by incubated in D-Hank's solution containing EGTA, the chelate of extracellular Ca2+. (3) The [Ca2+]i increase was not inhibited by pretreated nifedipine, Ca2+ channel blocker, but was abolished by pretreated with anthracere-9-cardoxylic acid (A9C), the Cl-channel blocker and which also blocked calcium activated non-selective cation channel (CAN).</p><p><b>CONCLUSIONS</b>These results suggest that the increase of [Ca2+]i induced by H2O2 of low concentration may be due to the influx of extracellular Ca2+ through CAN.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células Cultivadas
/
Cálcio
/
Microscopia Confocal
/
Hepatócitos
/
Peróxido de Hidrogênio
/
Canais Iônicos
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2002
Tipo de documento:
Artigo
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