Cytomegalovirus-specific T cells immune reconstitution after human leukocyte antigen matched sibling donor allogeneic bone marrow plus peripheral blood hematopoietic stem cell transplantation / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 605-609, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-278359
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regular pattern of Cytomegalovirus (CMV)-specific T cells (CTL) immune reconstitution after human leukocyte antigen (HLA) matched sibling donor allogeneic bone marrow(BM) plus peripheral blood hematopoietic stem cell (PBSC) transplantation.</p><p><b>METHODS</b>CTL from seventeen patients after transplantation was detected by flow cytometry, the IFN-γ secretion ability of CTL by enzyme-linked immunospot (ELISPOT) assay, and clonal analysis of TCR Vβ subfamily by gene scan assays. The relationship between CTL reconstitution and CMV infection was studied.</p><p><b>RESULTS</b>Both number and function of recipients CTL reached to normal control level at 30 d post-transplantation. The recipients achieved a high frequency CTL with IFN-γ response and restoration of T-cell receptor β (TCR Vβ) repertoire at one year post-transplantation. CTL with the central memory CD45RO(+)CD62L(+) cell phenotype expanded in PB when CMV was reactivated. The incidence of CMV reactivation was 35.83% (17.91% - 63.10%) after transplantation, and none of them developed CMV disease.</p><p><b>CONCLUSION</b>After HLA matched related donor transplantation using mixed grafts, immune recovery to CMV seems to be early and fast. The incidence of CMV infection and disease are low.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cirurgia Geral
/
Doadores de Tecidos
/
Linfócitos T Citotóxicos
/
Transplante de Células-Tronco Hematopoéticas
/
Citomegalovirus
/
Transplante de Células-Tronco de Sangue Periférico
/
Irmãos
/
Alergia e Imunologia
/
Doenças Hematológicas
/
Antígenos HLA
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2012
Tipo de documento:
Artigo
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