Effect of osteogenically and adipogenically differentiated bone mesenchymal stem cells from mouse on osteoclast formation / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1187-1190, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-278409
ABSTRACT
This study was purposed to investigate the regulatory effects of differentiating mesenchymal stem cells (MSC) on osteoclast formation. The MSC from mouse compact bones were cultured and induced into osteoblasts and adipocytes for one week. To test their regulatory effect on osteoclastogenesis, osteogenically differentiated and adipogenically differentiated MSC were co-cultured with CD11b(+) monocytes and osteoclasts were identified with in situ tartrate-resistant acid phosphatase (TRAP) staining. The results showed that differentiated MSC supported osteoclastogenesis but the osteoclast supporting capacity of osteogenically differentiated MSC decreased as compared with undifferentiated MSC. More interestingly, the adipogenically differentiated MSC significantly promoted osteoclasts formation when co-cultured with monocytes. It is concluded that the regulatory effect of MSC on osteoclast formation has changed while they have differentiated into different types of cells. The findings indicate that MSC may exert alternative effect on osteoclastogenesis by differentiation to descendant cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Osteoclastos
/
Células da Medula Óssea
/
Monócitos
/
Diferenciação Celular
/
Células Cultivadas
/
Técnicas de Cocultura
/
Biologia Celular
/
Adipogenia
/
Células-Tronco Mesenquimais
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2012
Tipo de documento:
Artigo
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