The study on methylation of gene IGSF4 promoter in acute leukemia cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 125-127, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-278784
ABSTRACT
To study whether gene IGSF4 was inactived by methylation in leukocytic cells, expression of IGSF4 was examined before and after treatment with demethylating agent in U937, Molt4 and HL-60 leukemia cell lines by means of RT-PCR. The methylation of promoter in U937, Molt4 and HL-60 cells as well as 21 acute leukemia patients was analyzed by MS-PCR. The results showed that methylation of IGSF4 promoter was inactived and could be reversed by treatment with a demethylating agent in U937, Molt4 and HL-60 cell. IGSF4 promoter methylation was detected in 57.1% of acute leukemia patients. There is no difference in incidence of IGSF4 promoter methylation between acute myelocytic leukemia and acute lymphocytic leukemia. In conclusion, IGSF4 is frequently inactived in acute leukemia and is a good candidate for the leukemia suppressor gene. As a normal suppressor gene, it may play an important role in inhibiting the development of leukemia, and the methylation of gene IGSF4 may be a good index in monitoring relapse of leukemia.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Imunoglobulinas
/
Leucemia
/
Moléculas de Adesão Celular
/
Reação em Cadeia da Polimerase
/
Doença Aguda
/
Regiões Promotoras Genéticas
/
Metilação de DNA
/
Proteínas Supressoras de Tumor
/
Linhagem Celular Tumoral
/
Molécula 1 de Adesão Celular
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo
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