Effect of inhibitors for demethylation and histone deacetylase on proliferation of cell line K562 and expression of tumor related genes / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 44-47, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-278804
ABSTRACT
In order to observe the effect of inhibitors for demethylation and histone deacetylase on the growth of leukemia cell line K562 and the expressin of tumor related genes, the K562 cells were treated with 5-aza-2' deoxycytidine (DAC) and trichostatin A (TSA) in co-culture; the growth curves were observed; the cell cycle was detected by flow cytometry (FCM); the gene expression pattern before and after drug treatment was measured with Atlas7742-1 microarray. The results showed that the combination treatment of DAC and TSA inhibited the proliferation of K562 cells, the growth of most cells were stopped in G(1)/S phases after drug treatment, the gene expression after treatment was more than before, and a few gene expression were down-regulated. In conclusion, combination treatment of DAC and TSA had an inhibitive effect on the leukemia cell line K562, combination of DAC and TSA with microarray could be used for screening candidate genes inhibiting leukemia cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Azacitidina
/
Ciclo Celular
/
Divisão Celular
/
Metilação de DNA
/
Células K562
/
Inibidores Enzimáticos
/
Inibidores de Histona Desacetilases
/
Ácidos Hidroxâmicos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo
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