Preliminary study on conversion of RhD positive red blood cells to RhD negative by modification with methoxy polyethylene glycol / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 654-658, 2003.
Article
em Zh
| WPRIM
| ID: wpr-278819
Biblioteca responsável:
WPRO
ABSTRACT
Rh is a very important blood group like ABO blood system in transfusion medicine. It causes severe transfusion reaction and hemolytic disease of the newborn (HDN) if RhD blood group does not match between the donor and the recipient. The population of RhD negative is only about 0.2% - 0.5% in Chinese. Conversion of RhD positive RBCs to RhD negative is very important in clinical transfusion. This study was to try to modify RhD antigen located on the surface of A, B, O and AB red blood cells in order to convert RhD positive to RhD negative by the modification of four kinds of methoxypolyethylene glycol (mPEG) derivatives and to observe the effect of mPEG modification on cell morphology, structure and function. The result demonstrated that modification efficiency of mPEG-BTC (mPEG-benzotriazole carbonate) was better than other three kinds of mPEG derivatives. It could camouflage RhD antigen efficiently when the concentration reached to 1 mmol/L. The result also showed that there were no harmful effects of mPEG modification on cell morphology, osmotic fragility, hemolysis, AchE, cholesterol, ATP, 2,3-DPG and deformability. It is suggested that success in converting RhD positive RBCs to RhD negative was preliminarily achieved.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Farmacologia
/
Fisiologia
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Polietilenoglicóis
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Sistema do Grupo Sanguíneo Rh-Hr
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Alergia e Imunologia
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Eritrócitos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Article