Camouflage of HLA-I antigen in lymphocyte surface / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 622-624, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-278826
ABSTRACT
The objective of this study was to investigate the method and effect of blocking the specific reaction between lymphocyte HLA-I antigen and its antibody. The lymphocytes were disposed with 12 mmol/L methoxypolyethelene glycol benzotriazol carbonate (mPEG-BTC) in concentration gradient in PBS (pH 7.4) at 22 degrees C. The effect of the modified lymphocytes was detected by microlymphocytotoxicity assay. The results showed that lymphocytes modified by mPEG-BTC did not react with related HLA-I antibodies in microcytotoxicity test. It is suggested that the specific reaction between HLA-I antigen of lymphocyte and HLA-I antibodies can be completely camouflaged by mPEG-BTC in PBS (pH 7.4) under 22 degrees C room temperature.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Polietilenoglicóis
/
Triazóis
/
Linfócitos
/
Antígenos de Histocompatibilidade Classe I
/
Citotoxicidade Imunológica
/
Alergia e Imunologia
/
Reações Antígeno-Anticorpo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
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