Antiproliferative effect of silencing mTOR gene on MCL Jeko-1 cell line and its mechanism / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 49-52, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-278916
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of silencing mTOR gene by RNA interference on proliferation and apoptosis, and its mechanism on mantle cell lymphoma Jeko-1 cell Line.</p><p><b>METHODS</b>The hairpin-like oligonucleotide sequences targeting mTOR gene was designed and transfected into Jeko-1 cells by lipofectamine TM 2000. The mTOR mRNA and protein were detected by RQ-PCR and Western blot. Cell growth was determined by MTT. Cell apoptosis was analyzed by flow cytometry. The expressions of Bcl-2, Bax, procaspase-3, procaspase-9, P70S6K,and p-P70S6K were detected by Western blot.</p><p><b>RESULTS</b>mTOR mRNA was markedly suppressed by shRNA targeting mTOR. mTOR shRNA suppressed proliferation and induced cells apoptosis of Jeko-1 cells. The cell apoptotic rates were (36.62 ± 3.24)%, (2.58 ± 1.04)%, (1.24 ± 0.30)% respectively, in mTOR shRNA, Neg-shRNA and Blank with statistically significant difference among them (P<0.05). mTOR shRNA down-regulated the expressions of Bcl-2, proCaspase3, proCaspase9 and p-70S6K, up-regulated the expression of Bax.</p><p><b>CONCLUSION</b>Deplete of mTOR gene may be realized through inhibiting the Akt/mTOR signaling pathway to promote the cell apoptosis and inhibit cell growth in Jeko-1 cell line.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Transfecção
/
Transdução de Sinais
/
Regulação para Baixo
/
Ciclo Celular
/
Apoptose
/
Linfoma de Célula do Manto
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Linhagem Celular Tumoral
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2015
Tipo de documento:
Artigo
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