Construction and expression of recombinant retrovirus vector carrying HBV vector / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 162-165, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-278988
ABSTRACT
<p><b>BACKGROUND</b>To explore the possibility of using retrovirus vector to carry HBV vector, and to prove that replication defective HBV could be normally packaged.</p><p><b>METHODS</b>Two kinds of full length of mutant HBV gene, which express dominant negative mutants, were inserted into retrovirus vector. After recombinant retroviruses were harvested, they were used to infect Hep G2 and 2.2.15 cell line. Then the expression of HBV core antigen in the Hep G2 cell was examined by immune fluorescence, and the existence of recombinant HB virion in the culture medium was examined by PCR.</p><p><b>RESULTS</b>High titer of recombinant retroviruses were obtained in the culture medium of transfected PA317 cell line. Core antigen was detectable in the recombinant retrovirus infected Hep G2 cell. Recombinant HB virion was detectable in the culture medium of recombinant retrovirus infected 2.2.15 cell.</p><p><b>CONCLUSIONS</b>The results suggested that recombinant retrovirus could carry HBV vector and express HBV products. When structural protein is offered by wt-HBV, the recombinant retrovirus may function as HBV vector, therefore it could be used in anti?HBV gene therapy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recombinação Genética
/
Retroviridae
/
Replicação Viral
/
Células Tumorais Cultivadas
/
Terapia Genética
/
Vírus da Hepatite B
/
Vetores Genéticos
/
Genética
/
Antígenos do Núcleo do Vírus da Hepatite B
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2002
Tipo de documento:
Artigo
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