Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>To search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.</p><p><b>METHODS</b>Quantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay.</p><p><b>RESULTS</b>Taqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower.</p><p><b>CONCLUSIONS</b>Sybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.</p>