Studies on the botanical sources and DNA molecular identification of Herba Asari based on ITS sequence / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 329-332, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-279171
ABSTRACT
<p><b>OBJECTIVE</b>To explore the genetic basis of using three species of Asarum as Herba Asari to determine the taxonomic positions of Asarum heterotropoides and A. siebodii; and to apply DNA molecular analysis as a tool for identification of Herba Asari.</p><p><b>METHOD</b>PCR, purification, sequence analysis were prerformed.</p><p><b>RESULT</b>MS sequences of the three Asarum species were obtained. 3 botanical sources of Herba Asari are closely clustered together on the topology tree; one inner branch is composed of A. heterotropoides and A. sieboldii, whereas another branch contains A. sieboldii. Their ITS sequences are different.</p><p><b>CONCLUSION</b>Three plant species of Herba Asari are closely related, and there are genetic reasons that they are used as the sources of the same medicine. The classification placement of A. sieboldii is not certain. The differences of ITS sequences of the botanical sources of Herba Asari can be used as a means of identification.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Plantas Medicinais
/
Especificidade da Espécie
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Classificação
/
DNA de Plantas
/
DNA Espaçador Ribossômico
/
Asarum
/
Genética
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2005
Tipo de documento:
Artigo
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