Determination of triptolide in Tripterygium total terpenoids tablets by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets.</p><p><b>METHOD</b>A Lichrospher CN column was used with ethanol and water for gradient elution. The detection wavelength was set at 255 nm.</p><p><b>RESULT</b>The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 microg x mL(-1) (r = 0.9998). The average recovery was 99.2%, RSD% = 1.7%.</p><p><b>CONCLUSION</b>The method is simple, rapid and accurate and can be used for quality control of the tablets.</p>