Intracellular dialysis with a microcatheter inserted into the patch-clamp pipette / 生理学报
Acta Physiologica Sinica
;
(6): 179-182, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-279315
ABSTRACT
In this paper we present an easily available method of intracellular dialysis via a microcatheter inserted into glass pipette during patch clamp experiment. An oblique hole through the glass pipette holder (above the lateral hole for cell-seal suction) is drilled, through which a microcatheter (O.D.=0.1 mm) made from the universal pipetter tip by hand-drawing passes and sticks out of the holder mouth in parallel with the Ag-AgCl electrode. With a syringe connected to the microcatheter, substitution of intracellular solution and intracellular dialysis of drugs can be achieved easily. Compared with repatch technique and intracellular solution substitution techniques used abroad, this method operates more easily and can produce more reliable results.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas de Patch-Clamp
/
Diálise
/
Desenho de Equipamento
/
Métodos
/
Microeletrodos
Idioma:
Chinês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2002
Tipo de documento:
Artigo
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