HPLC fingerprints of processed products from Sinapis alba / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2842-2845, 2010.
Article
em Zh
| WPRIM
| ID: wpr-279342
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba.</p><p><b>METHOD</b>The procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 254 nm, and the flow rate was 1.0 mL x min(-1). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was applied to analyze the similarity.</p><p><b>RESULT</b>The average similarities among the processed products were over 0.96, the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified.</p><p><b>CONCLUSION</b>The established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Medicamentos de Ervas Chinesas
/
Química
/
Química Farmacêutica
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Cromatografia Líquida de Alta Pressão
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Sinapis
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Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Article